"Detection of Antibiotic Resistance Genes in 40 Minutes"... On-site Diagnostic Technology Developed
National Institute of Health Research Optimizes CRISPR Technology
Demonstrates Potential for On-Site Molecular Diagnostic Applications
The National Institute of Health Research under the Korea Disease Control and Prevention Agency announced on June 10 that it has developed an on-site gene detection technology capable of identifying major antibiotic resistance genes of pathogenic bacteria at high risk of causing hospital-acquired cluster infections within 40 minutes.
This technology, which combines CRISPR gene-editing technology and gene amplification technology, enables the rapid on-site detection of antibiotic resistance genes without the need for complex specialized equipment. It is also characterized by its ability to completely prevent cross-contamination during the process.
Principle of Gene Detection Based on CRISPR/Cas12a. National Institute of Health Research
View original imageConventional gene amplification (PCR) tests are highly accurate but require specialized equipment and personnel, and have the limitation of long testing times. The research team developed a diagnostic method that performs both gene amplification and detection simultaneously in a single test tube, significantly simplifying the testing procedure and improving detection performance.
According to the research team, the new technology can detect major carbapenem resistance genes, such as KPC and NDM, even at very low concentrations. In evaluations conducted on actual patient samples, the technology demonstrated a sensitivity of 94.4% and a specificity of 98.7%. The entire process, from sample preprocessing to result confirmation, can be completed within 40 minutes, making it possible to conduct faster on-site diagnostics compared to existing testing methods.
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The research findings were published in the international journal 'Biosensors and Bioelectronics (IF 10.5)' and were also selected for the "Korea Scientists of Excellence (Hanbit-sa)" section by the Biological Research Information Center (BRIC).
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